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the samples were homogenized in a ball mill

the samples were homogenized in a ball mill; Effect of homogenization method on DNA yield and fragment size. Muscle homogenized cryogenically with the CryoGrinder or mortar and pestle were transferred to a container containing TEN9. Sample processing with rotor-stator and HT Homogenizer (bead beater) were homogenized in TEN9 buffer.

Application Note: Elemental Analysis: CHN

2017-8-11  Several coal samples with different CHN concentrations were analyzed to show the performance of the instrument. Samples were homogenized by a ball mill. To evaluate the repeatability of the system using argon as carrier gases, two Coal Reference Materials were analyzed. Samples were dried at 60 °C for two days.

Response of cytokinins and nitrogen metabolism in

2020-5-15  Phytohormones were extracted, purified and quantified according to previously published methods . In summary, the samples were homogenized with a MM301 ball mill (Retsch, Prague, Czech Republic) and stable isotope-labelled internal standards. Extraction occurred in cold (−20°C) methanol/water/formic acid (15:4:1, v/v/v).

Serine restriction alters sphingolipid diversity to

2020-8-12  The cells were homogenized with a ball mill (Retsch Mixer Mill MM 400) at 30 Hz for 3 min. Samples were centrifuged (14,000g, 4 °C, 10 min), supernatants were transferred to new tubes and

Physical activity reduces anxiety and regulates brain

2020-4-17  Deep-frozen samples were then homogenized in a ball mill to a fine powder and 50 mg of each preparation was lysed in 200 μL of 1 M triethylammonium acetate buffer containing 0.1% SDS. Four volumes of cold methanol were added to obtained lysates and samples were kept in − 20 °C for 30 min.

High abundance of Ralstonia solanacearum changed

2020-4-15  One part of the soil samples were homogenized in ball mill for 4 min at 45 Hz in 0.5 mL methanol solution (V methanol: V H2O = 3:1), then ultrasound treated for 5 min (incubated in ice water) for 5 times. The supernatant (0.4 mL) was transferred into a fresh centrifuge tube after centrifuged for 15 min at 10000×g at 4 °C.

AN42499 Elemental Analysis: CHNS/O Characterization

2020-9-16  a large range of element concentration. Solid samples were homogenized by a ball mill while liquid samples were analyzed without pre-treatment. Table 1 shows the nitrogen determination of pure solid samples. Instrument calibration was performed with urea (46.65 %N). Urea was analyzed also as unknown to check the accuracy and precision of the

What is the best way to homogenize small plant

The ball mill will be better for larger samples. Cite. 13th Mar, 2015. If you need to homogenized frozen plant tissue, it homogenized with dry ice using a vaFrozen riable speed laboratory

Hypogymnia Physodes Samples from U.S EPA Web Server

2012-2-21  Prior to digestion for multi-element analysis, lichen samples were ground and homogenized in a SPEX (Metuchen, NJ) 8000M ball mill equipped with zirconium balls. Grinding was performed at the University of Michigan laboratory of Dr. Joel D. Blum. Aliquots of homogenized lichen samples (25-35 mg) were digested in batches of 30-35 in a

What is Homogenized Milk? (with pictures)

2021-2-7  Homogenized milk is any sort of milk that has been mechanically treated to ensure that it has a smooth, even consistency. The homogenization process typically involves high temperatures, agitation, and filtration, all aimed at breaking down milk's

(PDF) The Influence of the Sintering Temperature on

The powders were homogenized in a high energy ball mill Pulverisette 6, cold compacted and then sintered using a Muegge type microwave heating installation. On these samples were carried out

Estudo de obtenção de revestimento de elementos

The samples were homogenized in a ball mill and the sintering temperatures were 1500°C and 2200°C, during times that varied of 30 until 240 minutes. The product was characterized by crystalline

verical mills for powdering ore samples

ebonite powdering machine. Mills for powdwering ore samples ebonite powdering machine amembazorgcentrum the samples were homogenized in a ball mill ficcifipicin mills for powdwering ore samplesximit the samples were homogenized in a milliso and ce grid type ore gold mining ball mill gold ore wet ball mill price read more ebonite powdering machine metal powdering machine

RESEARCH ARTICLE Open Access Comparison of freezing

2017-8-29  Leaf samples (100–200 mg) were homogenized with a ball mill, extracted in 1 ml of 0.2 N perchloric acid for 1 h at 4°C to extract free polyamines and centrifuged at 16000 x g at 4°C for 30 min. Since we detected only very low levels of bound polyamines in our samples (data not shown), these were not further investigated. To 100 μl

Utilization of Industrial Borax Wastes (BW) for Portland

BW (high boron content) samples after drying were homogenized in a ball mill. In the experiments, a 3-cell mold (4 x 4 x 16 cm) was used. In the rst stage, untreated BW was added to OPC at a ratio of 5% (w/w) and cement samples were formed by intergrinding OPC and untreated BW samples. At the second stage, mortars were prepared with 450 g

Response of cytokinins and nitrogen metabolism in

2020-5-15  Phytohormones were extracted, purified and quantified according to previously published methods . In summary, the samples were homogenized with a MM301 ball mill (Retsch, Prague, Czech Republic) and stable isotope-labelled internal standards. Extraction occurred in cold (−20°C) methanol/water/formic acid (15:4:1, v/v/v).

Geochemical analyses reveal the importance of

Dried sediment samples were homogenized and ground into a fine powder using a ball mill. The “Champagne test” [Jaschinski et al., 2008] was used to determine if samples contained inorganic C. Only four samples (all from WAP‐M core depths >60 cm) contained sufficient carbonate C to

Hypogymnia Physodes Samples from U.S EPA Web Server

2012-2-21  Prior to digestion for multi-element analysis, lichen samples were ground and homogenized in a SPEX (Metuchen, NJ) 8000M ball mill equipped with zirconium balls. Grinding was performed at the University of Michigan laboratory of Dr. Joel D. Blum. Aliquots of homogenized lichen samples (25-35 mg) were digested in batches of 30-35 in a

Quantitative determination of anthocyanins in three

2011-2-1  Then, they were homogenized in the aforementioned ball mill for 5 min. 2.4.3. FT-IR measurements. Infrared spectra of eight triplicate standards and ten triplicate samples of each sweet cherry variety were recorded. The spectra were collected with a Nicolet Magna 6700 FT-IR spectrophotometer equipped with a deuterated triglycine sulfate (DTGS

A comparison of homogenization methods of mouse

Mixer Mill (HT Homogenizer) (OPS Diagnostics, Lebanon, NJ) This high throughput bead beater grinds samples using stainless steel balls. Samples were homogenized in either deep well plates (DWP) with a 5/32" ball or 15 ml vial sets (15 VS) with two 7/16" balls. All samples were homogenized

A DNA based method to detect the grapevine root

2009-8-1  Samples were homogenized in a Ball-mill (Retsch MM301) at maximum speed for 2 min, spun down, and incubated shaking at 60°C for 10 min. 400 μl chloroform/isoamylalcohol (24:1) were added, samples were vortexed, centrifuged (5 min, 10000 ×g), and the aqueous top layer was transferred into a new 1.5-ml tube.

verical mills for powdering ore samples

ebonite powdering machine. Mills for powdwering ore samples ebonite powdering machine amembazorgcentrum the samples were homogenized in a ball mill ficcifipicin mills for powdwering ore samplesximit the samples were homogenized in a milliso and ce grid type ore gold mining ball mill gold ore wet ball mill price read more ebonite powdering machine metal powdering machine

Elemental Analysis: CHNS/O Determination in Carbon

2017-9-18  Different carbon samples were chosen to show the reproducibility obtained with the system. Coal, coke, graphite, lignite and carbon black samples were homogenized by a ball mill. Tables 1 and 2 show CHNS/O and CHNS determination of different matrices. Instrument calibration was performed with 2-3 mg of BBOT (2, 5-Bis (5-ter-butyl-

Response of cytokinins and nitrogen metabolism in

2020-5-15  Phytohormones were extracted, purified and quantified according to previously published methods . In summary, the samples were homogenized with a MM301 ball mill (Retsch, Prague, Czech Republic) and stable isotope-labelled internal standards. Extraction occurred in cold (−20°C) methanol/water/formic acid (15:4:1, v/v/v).

Physical activity reduces anxiety and regulates brain

2020-4-17  Deep-frozen samples were then homogenized in a ball mill to a fine powder and 50 mg of each preparation was lysed in 200 μL of 1 M triethylammonium acetate buffer containing 0.1% SDS. Four volumes of cold methanol were added to obtained lysates and samples were kept in − 20 °C for 30 min.

Utilization of Industrial Borax Wastes (BW) for Portland

BW (high boron content) samples after drying were homogenized in a ball mill. In the experiments, a 3-cell mold (4 x 4 x 16 cm) was used. In the rst stage, untreated BW was added to OPC at a ratio of 5% (w/w) and cement samples were formed by intergrinding OPC and untreated BW samples. At the second stage, mortars were prepared with 450 g

Effect of ball milling treatment on the Zn electrode

2020-6-18  as-prepared samples were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The conductivity of the Zn electrodes was examined by Potentiostatic Electrochemical Impedance Spectroscopy (PEIS) and it was found that the ball mill treatment of the electrodes with BSCCO additives increases their overall resistance.

Lipoxygenase6-Dependent Oxylipin Synthesis in Roots

Extraction samples were homogenized with a ball mill for 3 min at 20 Hz. After centrifugation, the supernatant was dried in a vacuum concentrator, and the extraction step was repeated with 1 mL ethyl acetate/formic acid (99:1, v/v).

Geochemical analyses reveal the importance of

Dried sediment samples were homogenized and ground into a fine powder using a ball mill. The “Champagne test” [Jaschinski et al., 2008] was used to determine if samples contained inorganic C. Only four samples (all from WAP‐M core depths >60 cm) contained sufficient carbonate C to

A High Phosphorus Diet Affects Lipid Metabolism in

2016-5-17  Freeze-dried rat liver samples were homogenized using a ball mill mixer (MM301; Retsch, Haan, Germany) for 3 min at 20 Hz with cooling using liquid nitrogen. Each 5 mg of homogenate was spiked with 100 μL of internal standard solution (1 mg/mL tridecanoic acid prepared in a 1:1 (v/v) solution of chloroform:methanol).